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Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea

Authors: Sunita Rao1 • Gyan Singh Shekhawat2

1Assistant Professor, Jaipur National University,Departmentof Biotechnology, Jaipur National University

2Professor, Department of Botany, Jai Narain Vyas University, Jodhpur, Rajasthan 342008, India

Name of Journal: 3 Biotech

Indexing: SCOPUS, Science Citation Index Expanded (SCIE)

Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea

Fig. 1(A) X-ray diffraction pattern of CuO NPs. (B) Morphological characterization of Copper (II) oxide nanoparticles using Scanning electron microscopy (X12,000) at a scale of 1 lM. (C) TEM micrograph of CuO NPs with an average diameter (33 ± 5) nm. (D) X-ray

Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea

Fig. 3 A(I) X-ray diffraction pattern of plant root samples exposed to CuO NPs. A(II) X-ray diffraction pattern of plant root TiO2 NPs. Marked arrows indicating the peaks d-spacing values in the root tissues

Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea

Fig. 2 (A) Graphical representation of effect of CuO NPs on shoot and root length of B. juncea. (B) Effect of CuO NPs (C, 1, 2, 3, 4) symbolize the plants at different concentrations, i.e., control, 200 500, 1000, 1500 mg/L resp. (C) Effect of TiO2 NPs on shoot and root length of B. juncea. (D) Effect of TiO2 NPs (C, 5, 6, 7, 8) correspond to the plants at different concentrations, i.e., control, 200 500, 1000, 1500 mg/L resp.

Phytotoxicity and oxidative stress perspective of two selected nanoparticles in Brassica juncea

Fig. 4 Effect of NPs on accumulation of H2O2 in leaves tested by means of the ROS sensitive dye DAB in B. juncea seedlings. a, e Control leaves showing no polymerized product as almost transparent visibility. b–dCuO treated plant leaves (200, 1000, 1500 mg/L) with greenish brown spots as compared to control plant leaves and f–h TiO2 treated plant leaves showing spots (200, 1000, 1500 mg/L), respectively

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